Somatic Embryogenesis from Cell Suspension Cultures of Taranoki (Aralia elata)

Synchronization of somatic embryogenesis in a carrot cell suspension culture.

Synchronization of somatic embryogenesis was achieved in a carrot (Daucus carota L. cv. "Kurodagosun") suspension culture by sieving the initial heterogeneous cell population, by density gradient centrifugation in Ficoll solutions, and by subsequent repeated centrifugations at a low speed (50g) for a short time (5 seconds), followed by transferring the cell clusters obtained, which were compose...

Four new saponins from the root bark of Aralia elata.

Four new saponins, 3-O-[beta-D-glucopyranosyl(1-->3)-alpha-L-arabinopyranosyl]-16a lpha-hydroxyoleanolic acid 28-O-beta-D-glucopyranosyl ester (called aralia-saponin I), 3-O-[beta-D-glucopyranosyl(1-->3)-alpha-L-arabinopyranosyl]-16a lpha-hydroxyhederagenin 28-O-beta-D-glucopyranosyl ester (aralia-saponin II), 3-O-[beta-D-glucopyranosyl(1-->3)-beta-D-glucopyranosyl(1-->3)-alpha-L-+ ++arabinopyr...

Somatic embryogenesis, embyogenic cell suspension from Ocimum sanctum (L.) leaf callus cultures and their rosmarinic acid accumulation

In vitro somatic embryogenesis from callus cultures of Cephaelis ipecacuanha

Callus was obtained from leaf explants of Cephaelis ipecacuanha on Murashige and Skoog (MS) basal medium supplemented with 0.5 mg/l Kin, 4.0 mg/l 2,4-D and 3% (w/v) sucrose [Murashige, T., Skoog, F., 1962. Plant Physiol. 15, 437±497]. Plantlet regeneration through somatic embryogenesis was achieved on MS medium containing 2.5 mg/l Kin, 1.0 mg/l 2,4-D and 3% (w/v) sucrose. Maturation and germina...

Somatic embryogenesis in Phoenix dactylifera: maturation, germination and reduction of hyperhydricity during embryogenic cell suspension culture

This work describes an improved and efficient method for optimum maturation and normal embryo growth to produce healthy plantlets through somatic embryogenesis using suspension cultures for date palm cv. sakkoty. cell suspensions were supplemented with low amounts of dichlorophenoxyacetic acid (0.5mg/1) and different concentrations of abscise acid (AbA) (0.1, 0.3 and 0.5 mg/l) and polyethylene ...